September 2015 SPADA Meeting

Dust Characterization

• Dust has been checked for cross reactive signatures with  MRI QA assays (LF, CapB, DHP7, chromosomal marker H1)‐ non‐reactive • Dust was checked for inhibition effects‐not inhibitory at  0.1 g using MRI extraction method • LOD  of spiked B. anthracis  in dust extract is 600 genome  equivalent copies (LF assay,) • Dust spiked with 600 spores tested positive for target (Ct=  36.7±0.45, N=50) 

Reference Dust (SRD)

Reference Dust

Dust Application Device

• Dust was pooled and dispensed into  244 × 50g aliquots.  Aliquots were  lyophilized, labeled and stored.

0.1g ±0.023g  for more  than 300 filters