September 2015 SPADA Meeting

Soils

• Soils were collected from five geographic locations  across the United States • Washington DC/Silver Spring • El Paso • Houston • Seattle • Kansas • Not all sites had all types of soils (loam, sandy loam,  sand, clay, subsoil) 

Soil Characterization S il if d d b i • o s were s te to remove e r s

• Tested for PCR inhibition extract from  a 1 g extraction  of soil‐ spiked with 1 pg; Spike blank N=1; Ct=34.9, soil  extract N=24; Ct=34.4±0.23 • No signature cross reactivity in the soils using MRI QA  PCR reagents for Bacillus anthracis • DNA sent to University of Houston for Metagenomic sequencing • Soils analysis characterization was completed • Soils were blended to make Master Lots