September 2015 SPADA Meeting

Background

 Legionellales, obligate intracellular parasite  G ti ith LPS Ph I d Ph II ram nega ve w : ase an ase  Metabolically active axenically, esp. at lower pH  Life cycle: LCV and SCV  Acute (>50% seroconvert-asymptomatic) and chronic infectious disease  Broad zoonotic reservoir; high seropositivity rate  Replicates in a “remodeled” phagolysosome-like vacoule  G i di ti * 2150 ORF enom c pre c ons : ~ s  Complete TCA, various aa auxotrophs  Large group of transporters  Proteomic skew to high pI  Complete Type 4 secretion element  >200 genes with single/point mutation “pseudogene”

Baca et al. 1984

*Seshadri et al. PNAS 2003

Background

Hundreds of Q fever cases reported during Operation Iraqi Freedom and Afghanistan war

* 15 JANUARY 2010 VOL 327 SCIENCE