Ultracentrifugation Catalog

www.centrifugebybeckman.com

Reference

Centrifugation is a basic separation technique that is utilized at multiple stages in the study of sample components. Flexible rotor and adapter systems for Beckman Coulter centrifuges allow them to be used across multiple application areas. To help you select the most appropriate centrifuge for your work, the following charts provide brief descriptions of the kinds of separations typically achieved using various centrifuges. These charts list frequent separation requirements for each sample type and identify the centrifuges that are typically used to meet those re­ quirements. Guide to Centrifuge Selection

In addition to the separation and isolation of sample particles, centrifu­ gation is increasingly being used as an analytical technique for the study of macromolecular interactions and the determination of molecular weights. Instruments for these applications are also listed below.

Quick-Reference Guide to Centrifuge Selection

Materials to be Isolated

Specific Application

Centrifuges Typically Used

Optima X Series

Optima MAX Series Airfuge J6 Series

Allegra Series Microfuge

Avanti Series

Preparative Centrifugation Proteins

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Ammonium sulfate precipitates Sucrose/glycerol gradient isolation Centrifugal filtration Fractionation by flotation Density gradient fractionation Fractionation by precipitation Chylomicron removal Sucrose gradient isolation Pelleting Sucrose gradient isolation Microsomal membrane fractionation Pelleting Sucrose gradient isolation Pelleting Pelleting Sucrose/Percoll gradient fractionation Binding studies Pelleting Size fractionation in sucrose gradients

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Lipoproteins

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Subcellular Fraction Chromatin/Nucleosomes Microsomes

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Mitochondria

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Nuclei

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Membranes

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Ribosomes/Polysomes

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Cytosol

Clarification

4-2

Beckman Coulter Ultracentrifuge