March 17, 2015-SP

Analyte Definition

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Description of the analyte: Determination of content of Vitamins B 1 , B 2 ,B 3 .  Total vitamin content consists of all free forms of each  nutrient and all common phosphorylated forms. ,and B 6 Common fortified forms are Thiamine chloride,  Riboflavin, Nicotinamide, and Pyridoxine. – Other common inherent forms are Thiamine mono, di  and triphosphate, FMN, FAD, Nicotinic Acid, Pyridoxal,  Pyridoxal 5’ phosphate, Pyridoxamine, and  Pyridoxamine 5’ phosphate.   Rationale:  –

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Specific Performance Claims B 1

Analytical range

30 – 2000 μg /100 g

Limit of Quantitation (LOQ) ≤ 30*

Recovery

90-110%

Repeatability (RSDr)

≤ 5%

Reproducibility (RSDR) ≤10%

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