March 17, 2015-SP

3/17/2015

“DIRECT” ANALYSIS METHOD

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March17,2015

MFA‐AOAC,2015 

LC‐MS CONDITIONS 

• The FDA publication used: • RP C 18 + ion pairing conditions (TBA) • APCI conditions, negative ionization mode • Single MRM transition

• Our approach •

Multi mode ODS column +non ion pairing conditions • ESI, negative ionization mode

•

Two MRM transitions

• Summation of two transitions for quantitation

Proprietaryand confidential — do not distribute © 2015 Abbott Laboratories

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MFA‐AOAC, 2015 

March17,2015

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