March 17, 2015-SP

3/17/2015

CHROMATOGRAPHY

• Multimode ODS column: •

Scherzo SS-C18 column (100 x 3 mm)* • Purified Porous Silica / Particle Size 3um / Pore Size 13nm / ODS + Anion Exchange + Cation Exchange Ligands • Separation achieved through a manipulation of eluent pH, organic solvent composition, salt concentration, and temperature. • Enables the retention of ionic species with LC-MS compatible mobile phases • Avoids the use of ion pairing agents in mobile phases

• Provides simple and rugged chromatographic retention

*= Imtakt USA, Portland, OR

Proprietaryand confidential — do not distribute © 2015 Abbott Laboratories

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MFA‐AOAC, 2015 

March17,2015

SAMPLE PREPARATION • For powders:

– Weigh 10.0  0.5 g of powder into a tared vessel and record mass. – Add water to a total weight of 70  5 g (powder + water) and record mass. – Stir until homogenous. • For ready-to-feed liquids and reconstituted powders: – Weigh 5.00  0.20 g liquid into a tared 15 mL centrifuge tube and record mass. – Add 40  L of internal standard working solution and vortex. – Add 50 μL of glacial acetic acid and 5.0 mL methanol, vortex. – Transfer aliquot to microfuge tube and centrifuge at 13,000 rpm for 10 min. – Filter an aliquot of the supernatant into an autosampler vial using a 0.2 um PTFE syringe filter.

Proprietaryand confidential — do not distribute © 2015 Abbott Laboratories

12

MFA‐AOAC, 2015 

March17,2015

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