SPIFAN Stakeholder Panel (March 15, 2017)

Summary of Method Validation Results for Whey Protein Analysis Using SDS-CGE (2) Parameter Acceptance criteria (SMPR) Results Specificity – Matrix Interference Electropherograms from injections of purified water and processed formulation matrix without protein ingredients will be evaluated for presence of peaks at the migration times corresponding to analyte proteins related peaks No interfering peaks were observed for purified water and processed formulation matrix

ч 10 whey protein g/100 g protein

Quantitation Limit

20%

Linearity

The R 2 must be •0.99. The residuals on the residual plot should be randomly distributed around zero.

Linear of R 2 0.993-0.999 for the area ratio of whey protein to casein Logarithm of R 2 0.993-0.996 for whey protein as % of total protein. Range – 20% to 80% of whey protein in total protein in infant formulas in tested linear range

Range

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Thank you!

The raw data of the SLV and the MLT of 4 Chinese labs are available upon request.

• Ping Feng, Wyeth Nutrition, 30F, CITIC Square, 1168 Nan Jing Rd. (W), Shanghai 200041, China Email: ping.feng@wyethnutrition.com ; phone: 18521400044 • Christophe Fuerer, Nestec SA, Lausanne, Switzerland; Email: Christophe.Fuerer@rdls.nestle.com • Adrienne McMahon, Wyeth Nutrition, Askeaton, Ireland; Email: Adrienne.Mcmahon@wyethnutrition.com The manuscript is being printed in the Mar/Apr 2017 Journal issue, 16-0344.R1 - Quantification of whey protein content in infant formulas by SDS-CGE (Sodium Dodecyl Sulfate – Capillary Gel Electrophoresis): First Action 2016.15 Single Lab Validation . The SLV is published on J. AOAC, the electronic version can be found with link: http://aoac.publisher.ingentaconnect.com/content/aoac/jaoac/pre-prints/content-16-0344

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