SPSFAM Allergens ERP

AOAC SPSFAM ERP REVIEW: MAIN FORM

Submission Date

2016-09-16 12:40:37

Name

Linda Monaci

E-mail

linda.monaci@ispa.cnr.it

Organization

CNR-ISPA

Title of Method

Multiplexed LC-MS method for the detection and quantitation of selected food allergens

AOAC Candidate Method Number (e.g. ALN-01)

ALL-02

Applicable SMPR

Yes

Summary:

The method submitted is a LC-MS/MS method for the multiplex detection and quantitation of milk, hazelnut, peanut and egg in different food matrices. The method proposed is based on protein extraction and subsequent digestion with three proteolytic enzymes (pepsin, trypsin, chymotrypsin); the final digest is partly purified before HPLC separation and MS/MS detection. The method was run on three different MS instrument: 2 triple quadrupole Mass Spectrometers in MRM mode and on a Q- TOF MS (selecting the precursor ion)

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes, the applicability of the method is in line with applicability of the SMPR.

Yes

Yes

Yes general precautions to be taken are specified.