SPSFAM Allergens ERP

4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method?

The method could be written more concisely without compromising the quality of the work performed.

Strengths:

The time required for the whole analysis appears to be shortened compared to the existing literature. The LODs reached are very challenging. The evaluation of the recovery has been done correctly and accruing the goodness of the method.

The possibility to extend the method also to other categories of nuts.

The method was successfully run on three different MS platforms.

6. Based on the supporting information, what are the cons/weaknesses of the method?

Weaknesses: The use of three different enzymes. It is expensive and add another variable to the whole procedure. Maybe a combination of two enzymes could be investigated to originate peptides with a medium length.

7. Any general comments about the method?

The method is very well described and the experimental work has been properly carried out. The final sensitivity and recovery calculated are very promising.

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

Yes with small modifications