SPSFAM Allergens ERP

4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. 1. Based on the supporting information, were there any additional steps in the evaluation of the method that indicated the need for any additional precautionary statements in the method? 3. Is there information demonstrating that the

The information about safety precautions redirect the operator to the consultation of MSDs for each single reagent used. No other information are provided about this aspect along the protocol.

No. In case of the starting material used for spikinf the food matrices (not specified the form and not taken into consideration the whole allergenic ingredient), the calculation of the recovery.

No the RM for allergens were not taken into consideration in this study. The use of CRM or incurred materials are strongly recommended in order to check method performance: e.g. recovery, etc.

The results provided in the table are not fully supported by the work performed, according to what shown and reported in the protocol. The experimental work carried out has not been properly detailed and some information are missing.

Yes especially when manipulating strong acids and MMTS (corrosive to tissues)