Breckland Scientific 2021 Catalogue

Electrophoresis

Electrophoresis Equipment

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GEL LOADING SOLUTION This concentrated solution is used to prepare nucleic acid and protein samples for agarose or polyacrylamide gel electrophoresis. Gel loading solution includes protein denaturants and the negatively charged tracking dye, Bromophenol Blue. Yields 5 ml final volume of DNA sample. Usage: Add 1 volume solution to 9 volumes of sample and mix well. Store at room temperature. 10x Concentration sufficient for 100 samples Cat No. Description Price Per BIO-870-020 Gel Loading Solution 15.80 1ml SYBR® SAFE Save time, money, the environment...and get better gel results! SYBR Safe® is a DNA stain that fluoresces with a bright green colour when excited with UV light. Like Ethidium Bromide, SYBR Safe® binds specifically to the DNA double helix. However, unlike Ethidium Bromide, SYBR Safe® has been engineered to be less mutagenic than Ethidium Bromide, making it much safer to use, particularly in the classroom. makes 750ml Cat No. Description Price Per BIO-870-029 SYBR Safe 60.28 makes 750ml is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It is the most sensitive stain available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment providing a safe , non-hazardous alternative to ethidium bromide. Store at 4°C up to 12 months, runSAFE Dye is light sensitive and should be stored protected from light. Cat No. Description Price Per BIO-885-012 Tracking Dye 35.85 1ml TRIS-GLYCINE-SDS BUFFER For protein gel electrophoresis. Enough powder to make 5L of 1x buffer. Store at room temperature Cat No. Description Price Per BIO-870-030 Tris-glycine-SDS Buffer 29.60 makes 5L TRIS-GLYCINE BUFFER The tris-glycine electrode buffer can be used for the electrophoresis of native, anionic proteins in polyacrylamide and agarose gels. Enough powder to make 5L of 1x buffer Cat No. Description Price Per BIO-870-032 Tris-glycine Buffer (10x Conc. for 5L) 29.60 makes 5L 1 BLUE LIGHT TRANSILLUMINATOR The SafeVIEW-MINI2 LED transilluminator offers the user a safe way to view and document samples, with the added benefit of an extremely uniform blue light source. This light source has the added advantage in that is does not damage DNA or RNA, unlike with UV light. With a large surface area, each transilluminator serves as the perfect workstation for viewing and working with fluorescently- stained protein and nucleic acid gels. Specifications Filter size: 15.4 x 15.4cm, Light source: 470nm Blue LEDs, Voltage: 110- 240V. Overall dimensions (W x L x H): 20 x 20 x 3cm. Weight: 1.52kg. Cat No. Description Price Per BIO-080-100 Cleaver Transilluminator 466.55 ea. TRACKING DYE RunSAFE is a non-mutagenic fluorescent reagent that produces instant visualisation of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, CSL-runSAFE

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Cleaver Blue Light Transilluminator

Electrophoresis Reagents

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AGAROSE Cat No.

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Description

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BIO-350-050 Agarose (5g)

4.58 5g

BIO-350-120 Agarose (4 x 5g packs)

16.32 20g

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AGAROSE - LOW MELTING POINT An environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures. A low EEO flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved, while a high gel strength aids handling and maintains compatibility with blotting techniques. This low melting point form is ideal for nucleic acid recovery and enzymatic applications, as well as in a high-resolution PCR-grade form to resolve very

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small nucleic acid fragments 20-800bp in size. Melting point: 65oC; Gelling point: 26-30 oC Cat No. Description

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Price Per

BIO-375-100 Low Melting Point Agarose 5g

24.10 5g

BIO-375-200 Low Melting Point Agarose 50g

213.35 50g

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DNA MARKERS Pre-made and containing loading dye for immediate use, these ready-to-use DNA Markers are specially formulated to run accurately and produce sharp, well defined ladders. Available in six molecular weight ranges and composed of discrete marker fragments isolated from restriction- digested proprietary plasmids, each DNA marker will remain stable for up to 6 months at room temperature and 12 months if kept in the fridge at 4°C. Cat No. Description Price Per BIO-890-014 1Kbp DNA ladder, 250-10Kb 24.05 500µl BIO-890-016 Broad Range DNA Ladder, 100bp-10Kb 45.28 500µl ELECTROPHORESIS BUFFER This powdered blend Tris-Borate EDTA buffer (TBE), has been optimised for agarose gel electrophoresis of nucleic acids. Dissolve powder in 500 ml of distilled or deionised water to prepare a 10x concentrate which can be diluted 10-fold to make a 1x working electrophoresis buffer. Powdered Blend for 5 litres. Store at room temperature. Also available as a ready-made-up solution. Cat No. Description Price Per BIO-870-036 Electrophoresis Buffer (10x Conc.) 39.47 500ml BIO-400-100 Electrophoresis Buffer Solution (50x Tae) 39.45 100ml

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