Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Title

VALIDATION OF B-COMPLEX VITAMINS

Test Parameters

Thiamine, Riboflavin, Niacinamide, Nicotinic acid, Pantothenic acid, Pyridoxine, Biotin, Folic acid

Reference

AOAC -2012 - APPENDIX L, K

AOAC Recommended Guideline for Stake holder Panel on Infant Formula and Adult Nutritionals (SPIFAN) SANCO-12571-2013 : Guidance document on analytical quality control and validation procedures for pesticide residues analysis in food and feed.

Products Selected

Infant Formula- Lactogen Infant Formula - Cerelac

Eezee Paste Fruit Juices

Parameters

No. of Runs

Replicates

Rule

Linearity/Calibration Fit

6

1

LOD

10

1

LOQ

10

1

Specificity

Blank

6 1

1 1

RT Ratio = 0.95 to 1.05 Ion Ratio = Refer Table

Sample (1849a)

Precision

Repeatability (same analyst, same day)

Day-1

RSD & Horrat - Refer Table Fresh Standards & Fresh Reagents every batch

5

1

Day-2 5

Reproducibility -(different -days/analysts)

1

SRM 1849a

5

1

Accuracy (Trueness) -

SRM 1849a

5

1

Recovery % - Refer Table Page 3