Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

Test Group Name

B-complex Vitamins Thiamine Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

2000000.00

Calibration Standards

Concentration (ug/L)

Area-Analyte

y = 3850.1x - 22469 R² = 0.9993

5.00

8453.63

1500000.00

Solvent Standard Level-1

20.00

53305.80

Solvent Standard Level-2

1000000.00

100.00

340778.96

Solvent Standard Level-3

200.00

737865.01

Solvent Standard Level-4

500000.00

300.00

1164641.72

Solvent Standard Level-5

0.00

500.00

1891466.35

Solvent Standard Level-6

0.00

100.00 200.00 300.00 400.00 500.00 600.00

-500000.00

R 2 ≥ 0.99

Criteria

Conclusion

Complies

Page 13