Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Validation Parameter

Limit of Quantification

Test Group Name

Date

TUV/BLR/F-360 2015\January\Vitamins\01.01.2015 01.01.2015 Prasad BKVR/Kesav Kumar N Manjunath K/Prasad BKVR TUV/BLR/F-99

B-Complex vitamins Thiamine Aqueous

Test Name

Data Path File

Matrix

Raw-Data File Ref. CRM Data File Ref.

Limit

Equipment Test Method

Analyst

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

Operator

Repeatability at LOQ 5 ug/l standard Sample Blank No.

Mean Retention Time (min)

m/z

Signal (ug/l)

Caluclation

265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2

1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823

4.608 4.785 4.453 4.814 4.194 4.135 4.675 4.774 4.099 3.911

1 2 3 4 5 6 7 8 9

Mean (μg/l)

4.44

SD

0.3339

LOD (Mean +3 sigma) LOQ (Mean + 10sigma)

5.45 7.78 7.51

RSD %

5

Standard Conc. (μg/l)

88.9

Accuracy %

Accuracy = 70 - 125 %

Criteria

10

RSD% = <15%

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