Review Team (February 2016)

VitD-18 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 1: Accuracy—Recovery

Spike Recovery

Replicates (n)

Product

SPIFAN Code

Analyte

+ Spike Level 1

+ Spike Level 2

Native level

Average

Average

RSD

RSD (%)

1.6 (IU hg -1 ) 2.4 (IU hg -1 ) 0.5 (IU L -1 ) 3.6 (IU L -1 ) 3.0 (IU L -1 ) 0 (IU hg -1 ) 0 (IU hg -1 ) 0 (IU L -1 ) 0 (IU L -1 ) 0 (IU L -1 ) Global Mean

135 (IU hg -1 ) 136 (IU hg-1) 269 (IU L -1 ) 270 (IU L -1 ) 268 (IU L -1 ) 198 (IU hg -1 ) 191 (IU hg-1) 389 (IU L -1 ) 403 (IU L -1 ) 375 (IU L -1 )

97% 285 (IU hg -1 ) 98% 276 (IU hg -1 ) 98% 555 (IU L -1 ) 98% 559 (IU L -1 ) 97% 547 (IU L -1 ) 100% 408 (IU hg -1 ) 96% 395 (IU hg -1 ) 98% 789 (IU L -1 ) 101% 813 (IU L -1 ) 94% 789 (IU L -1 )

Child Formula Powder Infant Elemental Powder

00847RF00

3 3 3 3 3 3 3 3 3 3

101%

00796RF

98% 99% 99% 97%

Vitamin D 2

Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat Infant Formula RTF, Milk-Based

00821RF00 00820RF00

EV4H2Q

Child Formula Powder Infant Elemental Powder

00847RF00

101%

00796RF

98% 98%

Vitamin D 3

Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat Infant Formula RTF, Milk-Based

00821RF00 00820RF00

101%

EV4H2Q

98% 99%

98%

Additional information to be completed by SD

Were products weighed into extraction vessels before spiking? Were products spiked before they were weighed into extraction vessels? Was a portion of the product spiked? Were all spike recovery samples taken from the same spiked product? Were multiple portions of the product spiked and each spiked product analyzed? Were all spike recovery samples prepared on multiple days? Were spike recoveries calculated from theoretical additions? Or was spiking solution analyzed and the results used to calculate spike recoveries? What form of the nutrient was spiked into the product? What kind of solution or solvent was used to deliver the spike to the product? Were all spike recovery samples prepared on the same day?

yes yes no yes

no no

yes no yes

vitamin D 2

and vitamin D 3

Ethanol 0.5% (50 μL in 10 mL)

What percentage of the spiked sample was the spiked was the spike blanc? (How much was product diluted by spike?)

SPIFAN ERP Checklist v1.0