SPIFAN SP Meeting September 17, 2016

Folate SMPR 2011.006 (approved April 5, 2011)

Applicability: Determination of total folate [supplemental folic acid or 5-methyl-tetrahydrofolate, and endogenous 5-methyl-tetrahydrofolate polyglutamate] in all forms (powders, ready-to- feed liquids, and liquid concnetrates) of infant, adult, and pediatric nutritional formula.

SPIFAN Stakeholder Panel September 2010

UPLC/MS/MS procedure 13 C-Folic Acid (internal std)

Protease

Amylase

Homogenize

Buffer & Heat

T. Gbatu, J. Szpylka, A. Gheney, AOAC Annual meeting 2008.