SPIFAN SP Meeting September 17, 2016

Ion Chromatography Method (Fluo-02) ThermoFisher Scientific. Analysis of fluoride in Infant Formula and Adult Nutritionals by ion chromatography after ultracentrifugation • Good linearity (> 0.995) and estimated LOQ (≈ 5μg/100g) • Good RSDr (< 5%) , RSDR (<10%) and recovery (81%-105%) for 12 SPIFAN products • Specific equipment and IC consumables (column, cartridges, buffer) needed / Chloride analysed simultaneously Conclusion: equipment dependent / method to be improved for low F level (e.g. removal of organic acids, column and buffer used, sample volume injected) for a better chromatogram interpretation / Complete SLV & additional SLV chloride could be a plus using improved method

September 2016

9

Reference 10 is listed in slide 16

Microwave acid digestion and F-ISE 10

• Alternative to tedious & time consuming alkali fusion or microdiffusion • LOQ blank: 5 μg/L / LOQsample ≈ 20 μg/100g dry matter (1g with DF=2 TISAB 20%) / 20 food samples (seafood, vegetables, fruits: 0.08 – 1.8 mg/100g dry matter) • Not validated for infant formula: (estimated LOQ :< 20 μg/100g RTF) Conclusion: feasibility to test for Infant Formula with some modifications (e.g. diluted HNO 3 / 2g IF reconstituted / F-ISE calibration type) 10 September 2016