BPS Program Book 2014

5:00 pm –8:00 pm , R oom 307 Korean Biophysicists Meeting 6:00 pm –7:00 pm , R oom 121 Biophysics Austria Mixer 6:00 pm –7:30 pm , R oom 302 Biophysical Society of Canada– Travel Awards and Mixer 6:00 pm –9:00 pm , H all D Student Research Achievement Award (SRAA) Poster Competition This session features students who are presenting posters at the Meeting and have pre-registered for the competition. During the competition, students give a five-to-seven minute verbal presentation of their poster to one or more judges. Winners will be recognized on Monday evening prior to the National Lecture. 7:00 pm –8:30 pm , R oom 123 Exhibitor Presentation FEI Company Cryo-TEM: A New Era for 3D Structural Analysis of Protein Complexes A new frontier exists in unraveling interactive biological and biochemical processes and pathways at the macromolecular level. Of critical impor- tance is the three-dimensional visualization of macromolecular structures and molecular machines in their native functional state. Three techniques play a major role in orchestrating this. Nuclear magnetic resonance (NMR) has the capability to study specific protein domains or fragments and their functional role in protein fold- ing and dynamics and in ligand binding whereas X-Ray crystallography (XRD) allows visualizing high-resolution but more static 3D structures of apo and liganded proteins, mainly in a monomeric or dimeric state after crystallization. To unravel more physiologically relevant situations how- ever, it is essential to visualize multimeric complexes in their tertiary and quaternary state and their interaction with other complexes. By perform- ing typical cryo-TEM applications like single particle analysis or tomog- raphy, this can be achieved. In this so-called translational methodology, cryo-TEM thus provides complementary information to NMR and XRD that can be crucial for drug discovery, e.g. in terms of a better understand- ing of the mechanism of action inferred from the EM structure of the physiologically relevant complex. This will eventually contribute to answer real biologically as well as medically relevant questions. Latest develop- ments in the cryo-TEM workflow have brought the three major structural biology technologies closer together. Now, finally, a continuum has been reached on all important aspects with regards to resolution and macromo- lecular scales which allows for the full deployment of the combination of these technologies. Here, we will illustrate the historical context of these technologies with respect to one another and show how latest developments have reached the critical requirements to fully unleash the power of structural biology in not just answering fundamental questions, but actually contribute to curing diseases and improving health. Also, we will discuss the future of structural biology based on the latest developments of the FEI workflow and its components. Presenters: Marc Storms, Marketing Manager, Life Sciences, FEI Company Jeff Lengyel, Product Marketing Manager, FEI Company Eric Hnath, Product Marketing Manager, Structural Biology, FEI Company Thomas Wohlfarth, Director, Structural Biology Businesses, FEI Company

231-P lat 4:15 pm COMPRESSION, VOLUME AND PROLIFERATION ARREST. Morgan Delarue , Fabien Montel, Danijela Vignjevic, Jacques Prost, Jean-François Joanny, Giovanni Cappello 232-P lat 4:30 pm THE EVOLUTION OF MECHANICAL PROPERTIES OF DIFFERENTIATING STEM CELLS IS FATE- AND FUNCTION- DEPENDENT. Andrew Ekpenyong, Jochen Guck 233-P lat 4:45 pm VIEWING NUCLEAR DEFORMATION WITH SIDEWAYS MICROSCOPY. Kellie N. Beicker , Timothy E. O’Brien, Michael R. Falvo, Richard Superfine 234-P lat 5:00 pm IMAGING MECHANICAL FORCE TRANSMISSION AT SINGLE INTEGRINS IN LIVING CELLS. Masatoshi Morimatsu , Armen H. Mekhdjian, Alice Chang, Alexander R. Dunn 235-P lat 5:15 pm THE ROLE OF ARP2/3 IN DRG GROWTH CONES MOTILITY. Wasim A. Sayyad , Paolo Fabris, Jelena Ban, Erika Ercolini, Vincent Torre 236-P lat 5:30 pm PROBING CELL MEMBRANE MECHANICS BY MAGNETIC PARTICLE ACTUATION AND 3D ROTATIONAL PARTICLE TRACKING. Matthias Irmscher, Arthur M. de Jong, Holger Kress , Menno W.J Prins 237-P lat 5:45 pm TIGHT COUPLING BETWEEN NUCLEUS AND CELL MIGRATION THROUGH THE PERINUCLEAR ACTIN CAP. Dong-Hwee Kim , Denis Wirtz 5:00 pm –6:30 pm , R oom 123 Exhibitor Presentation Asylum Research, an Oxford Instruments Company New blueDrive™ Photothermal Excitation for Superior AFM Tapping Mode Imaging Asylum Research, an Oxford Instruments company, will introduce its new blueDrive Photothermal Excitation capabilities exclusively avail- able on Cypher™, the highest resolution fast scanning AFM. blueDrive significantly enhances the performance of tapping mode imaging with more simple, stable and quantitative operation, and providing extremely clean tunes in both air and water. Typically, a piezoacoustic excitation has been used to drive the cantilever oscillation. Though piezo drive is favored for design simplicity, the response of the cantilever is often far from ideal, causing users to spend countless time selecting a clean canti- lever tune. Asylum’s blueDrive excitation mechanism produces an almost perfect response by directly exciting the cantilever photothermally with a blue laser. blueDrive is ideal for high resolution imaging of biological samples in fluid including proteins, lipids and nucleic acids, as well as force measurements and nanomechanics. In this presentation, we will explain how blueDrive works, how it achieves simple cantilever tunes, and show real world results for biophysics applications. Presenter: Nick Geisse, Applications Scientist, Asylum Research, an Oxford Instruments company

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Biophysical Society 58 th Annual Meeting, San Francisco, California