Biophysical Society Thematic Meeting | Singapore

Mechanobiology of Disease

Poster Abstracts

54-POS Board 54 Magnetic Control of the Gating of Bacterial Mechanosensitive Channel Mscl for Smart Liposomes Yoshitaka Nakayama 1 , Mislav Mustapic 2,3 , Haleh Ebrahimian 1 , Pawel Wagner 2 , Jung H. Kim 2 , Md Shahriar Hossain 2 , Josip Horvat 2 , Boris Martinac 1,4 . 1 Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia, 2 University of Wollongong, North Wollongong, NSW, Australia, 4 University of New South Wales, Darlinghurst, NSW, Australia. 3 University of Osijek, Osijek, Croatia, Liposomal drug delivery systems (LDDSs) are effective tools used for the treatment of diseases especially when highly toxic pharmacological agents are utilized. In order to regulate the drug release from liposomal capsules, remotely controlled nanovalves with a large conductive pore are required. The bacterial mechanosensitive channel of large conductance, MscL, presents an excellent candidate biomolecule for the LDDSs. In this study, we developed superparamagnetic nanoparticles for the activation of the MscL nanovalves by magnetic field. Synthesised CoFe 2 O 4 nanoparticles with the radius of less than 10 nm were labelled by SH-reactive chemical groups for attachment to M42C MscL mutant. Activation of MscL by magnetic field with the nanoparticles attached was examined by the patch clamp technique showing that the number of activated channels under ramp pressure increased upon application of the magnetic field. In parallel, we have tested and have not observed any cytotoxicity of the nanoparticles in human cultured cells. Our study suggests the possibility of using magnetic nanoparticles as a specific trigger for activation of MscL nanovalves for drug release in LDDSs.

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