AOAC SPADA February 2015 Meeting Book

Annex III: Non-Target Panel

A suitable non-target panel shall be selected based on the analytical technique.

For non-bioassays: it is expected that an appropriate non-target panel will be developed for the selectivity study in Table 2 of the performance requirement table. For a bioassay based on PDE5 inhibitors: natural components/matrices with PDE5 inhibition activity shall be evaluated, such as extracts/powders of Tribulus terrestris, Cnidium monieri, Morinda officinalis, Cuscuta chinensis, and Epimedium koreanum. For immunoassay, structurally similar compounds should be tested, such as the following examples of compounds that are subcomponents of PDE5 inhibitors, which are provided for illustration purposes: 2-Pyrimidinecarboxamide 5-pyrimidinecarboxamide Benzenesulfonyl Piperazin Piperazinyl Piperazinylphenolate 3,6,7,12,12a-hexahydro-2-methyl-pyrazino [1', 2':1,6] pyrido[3,4-b]indole-1,4-dione Benzenesulfonamide