AOAC SPADA February 2015 Meeting Book

Fitness for Purpose (proposal)

Id tifi ti

en ca on o 

f VEEV i

i  n env ronmen a  t l

samples The method must be able to identify VEEV RNA  resulting from aerosol, solid or liquid samples.   Ideally, the method should include only epizootic  VEEV (Subtypes 1AB, 1C) and should exclude all  other VEEV.  The limit of detection must be lower  than one infectious human dose of VEE. 

Fitness for Purpose (proposal)

Id tifi ti

en ca on o 

f VEEV i

i  n env ronmen a  t l

samples The useable matrices for the PCR test (aerosol  collections, environmental swabs, air filters, etc.)  are defined with respect to their complexity,  presence of organic material and inorganic  material, and the expected duration of detectable   alphavirus RNA by PCR.