AOAC SPADA February 2015 Meeting Book
STAKEHOLDER PANEL ON Agent detection assay:PCR Background & Fitness for Purpose Coxiella burnetii
James E. Samuel, PhD Professor and Chair, TAMHSC February 3, 2015
Legionellales, obligate intracellular parasite G ti ith LPS Ph I d Ph II ram nega ve w : ase an ase Metabolically active axenically, esp. at lower pH Life cycle: LCV and SCV Acute (>50% seroconvert-asymptomatic) and chronic infectious disease Broad zoonotic reservoir; high seropositivity rate Typical route of infection via aerosol of contaminated soils R li t i “ d l d” h l lik l ep ca es n a remo e e p ago ysosome- e vacou e Genomic predictions * : ~2150 ORFs Complete TCA, various aa auxotrophs Large group of transporters Proteomic skew to high pI Complete Type 4 secretion element >200 genes with single/point mutation “pseudogene” *Seshadri et al. PNAS 2003 Baca et al. 1984
Made with FlippingBook