AOAC SPADA February 2015 Meeting Book

Mouse sublethal challenge model

Infected mice:IP Wild type mice relatively resistant to clinical disease but readily colonized: splenomegaly correlates with bacterial load Aerosol and oral challenges models developed for virulence and protection studies

Zhang et al. IAI 2004

1 4 0 0.2 0.4 0.6 0.8 1 1.2 .

Spleen Weight

0.E+00 1.E+05 1.E+06 1.E+07 1.E+08 1.E+09

C. burnetii challenge dose (Log 10 )

*

*=p<0.05 **=p<0.01

Mouse Infection-derived Immunity: IP Challenge

Spleen Weight

Whole Spleen Cb Copy #

1 0 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 . Average Spleen Weight (g) **

1 0 10 7 . _

**

1.0_10 6

1.0_10 5

*

1.0_10 4

Whole Spleen

1.0_10 3

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

Average Genome Copy # per

Group

Group

1 2 3 4 5 6 7 8

Negative control 10 6 Cb inf (3 wk)

10 6 Cb inf (3 wk), Doxy (2 wk), rest (1 wk)

10 6 Cb inf (6 wk)

10 6 Cb inf (3 wk), Doxy (2 wk), rest (1 wk), 10 7 Cb inf (2wk)

10 6 Cb inf (6 wk), 10 7 Cb inf (2wk) Non-inf (6 wk), 10 7 Cb inf (2wk) pI vax (6 wk), 10 7 Cb inf (2wk)