AOAC SPADA February 2015 Meeting Book

Natually obligately intracellular Recent advances in axeminic media (ACCM2)

Intracellular lifecycle

Recent genetic and mutant

analysis

van Schaik et al. Nat Rev 2013 Primary virulence tools include T4SS and T2SS effectors

Comparative genomics: Pathotype model

Group(*)Plasmid type(&)Isolate(+)

Phase($)Original source

Disease

Passage([[)

I

QpH1

NineMile RSA493

I

Montana tick, 1935 Montana tick, 1935 Montana tick, 1935 Montana tick, 1935

- - - -

307GP/1TC/1EP 90EP/1TC/1EP 4 EP/306GP/3EP 4 EP/306GP/3EP

NineMile RSA439(>) II NineMile RSA514(>) I/II# NineMile RSA285-A(>)I/II# American Q Dyer(>) I Australia QD RSA425 II Dyer RSA 345 II

USA, humanblood, 1938 USA, humanblood, 1938 Australia, humanblood, ~1939 Turkey, humanblood, 1948 Central Africa, humanblood, 1949 Central Africa, humanblood, 1949

Acute Acute Acute Acute

81EP

75EP/1GP

177EP 31EP

Turkey RSA333 African RSA334(>) Giround RSA431(>) ElTayeb RSA342 Panama RSA335

II

I I I I

Acute, Congolese RedFever3HP/4EP Acute, Congolese RedFever2GP/2EP

Egypt, tick, 1967

- -

4GP/2EP

Panama, chiggers, 1961 California, cow'smilk, 1947 California, cow'smilk, 1947 Ohio, cow'smilk,1956 Ohio, cow'smilk,1956 Italo-Greek, 'Grita', ~1945 Italo-Greek, 'Grita', ~1945 Italy, humanblood,1945

4EP 6EP

California 33 RSA329 I California 16 RSA350 II

Persistent Persistent Persistent Persistent

38EP 4EP 42EP ?/2EP 36EP 2EP

Ohio 314 RSA270 Ohio 314 RSA338

I

II II II II

II

QpH1

M44 RSA459 M44 Q141(>)

Acute Acute Acute

?/1GP/2EP

Henzerling RSA331 Idahogoat Q195

III

QpH1

I I I I I I I I I I I I I I I I

Idahogoat,1981

Abortion Abortion

Idahogoat

Idahogoat placenta, 1975

5EP

Koka

Ethiopia, tick, 1963

-

1GP/6EP GP/2EP 1GP/1EP 1GP/4EP HV/2EP HV/2EP HV/3EP HV/2EP HV/2EP HV/2EP BX/2EP HV/2EP

IV

QpRS

MSU GoatQ177 Canada GoatQ218 Idaho Sheep 80-1

Montana,goat cotyledon,1980

Abortion

Ontario, Canada,goat spleen,1981 Abortion

Idaho sheep liver,1980

Abortion

KQ154 PQ173 FQ228 LQ216 GQ212 SQ217 KoQ229 HWSU101

Oregon, human heart valve,1976

Endocarditis

California, humanheart valve,1979 Endocarditis Washington, humanheart valve, 1982Endocarditis California, humanheart valve,1986 Endocarditis NovaScotia, humanheart valve,1981Endocarditis NovaScotia, humanheart valve,1981Endocarditis Montana, human liverbiopsy, 1981 Hepatitis NovaScotia, humanheart valve,1982Endocarditis

V

NP

VI

QpDG

Dugway 7E22-57 Dugway 7E9-12

Utah, rodents, 1958 Utah, rodents, 1958

- -

3EP 3EP

Samuel et al. 1985