AOAC SPADA February 2015 Meeting Book

C. burnetii phylogenetic organization

SNP and VNTR based trees for 25 worldwide isolates of Coxiella burnetii. Geographical distribution shown below. Pearson, Keim et al. ASM2005

Glazunova et al. EID 2005

C. burnetii – Existing PCR assays  • Purpose: Detect C. burnetii DNA in  clinical and environmental samples – Environmental samples, milk, soil, animal  Tissues, air  sampling – Human blood samples • Most common targets:   IS1111, mulit‐copy IS element – Range in copy number among isolates (~20)  Com‐1: encodes outer membrane (DsbA/C) protein – Highly conserved among isolates • qPCR method  – Brennan et al (IAI 2003) – Sensitivity approaches one genome equivalent