AOAC SPADA February 2015 Meeting Book

Standard Method Performance  Requirements ‐ Goal

Development of Standard Method Performance  Requirements (SMPR) for Coxiella burnetii by  PCR method, with the possibility of developing a  single SMPR for the detection of the diversity of  isolate variation 

Fitness for Purpose (proposal) Identification of C. burnetii in environmental  samples The useable matrices for the PCR test (aerosol  collections, environmental swabs, air filters, etc.)  are defined with respect to their complexity,  presence of organic material and inorganic  material, and the expected duration of detectable   DNA by PCR.