AOAC SPDS Meeting Book March 19 2015

Analytical Challenge • Traces of latex containing aloin may be present in inner  leaf juice production, or substantial amounts (several  percent) are found in leaf juice prior to removal, usually  via activated carbon filter • Very low concentration (ppb/ppt levels) in purified aloe  vera juice and dry juice ingredients and finished products  R l t d d / th i l t d • e a e compoun s an ocyan ns can co‐e u e an can  lead to misinterpretation • Need cost effective test method to find trace levels  (ppb/ppt)

Current analytical methods and techniques Currently four analytical techniques are available for aloin  testing: • Thin Layer Chromatography: For identification TLC does  not provide quantitative results for low levels (ppb/ppt) • HPLC: Good resolution, cost effective, easy sample  preparation, most labs can run HPLC • LC‐MC and GC‐MS: good resolution, expensive  instrumentation and maintenance as compared to  LC/HPLC, need trained chemist for interpretation