AOAC SPDS Meeting Book March 19 2015

STAKEHOLDER PANEL ON Dietary  Supplements Background & Fitness for Purpose Vitamin D

John Austad d AOAC 2015 Mi ‐Year Meeting Rockville, MD 19 March 2014

Background on analyte

• Vitamin D 

– Two Primary Forms: • Ergocalciferol (Vitamin D 2 )

– Formed from UV‐Light Irradiated Ergosterol – C 28 H 44 O  Molecular Mass = 396.55 g/mol Ch l l if l (Vit i D ) • o eca c ero am n  3 – Formed from UV‐Light Irradiated 7‐dehydrocholesterol – C 27 H 44 O  Molecular Mass = 384.64 g/mol