AOAC SPDS Meeting Book March 19 2015

Methods (FDA)

 FDA Method

Spectra and Q/Confirmation Ions of Powdered Kratom Leaf

Abundance

Scan 1706 (14.649 min): 07071410.D 397 214

1200000 1400000 1600000 1800000 2000000 200000 400000 600000 800000 1000000

269

186

367

75

155

327

129

240 295

103

429 479 553 505 589619647

50 100 150 200 250 300 350 400 450 500 550 600 650 0

m/z-->

Challenges

• GC Methods – not commonly applied for M. speciosa , particularly in the wide  variety of matrices encountered – High temperatures required to elute the alkaloids – restricts  parameter adjustment for resolution of any alkaloid mixture – inadequate resolution of mitragynine and speciociliatine – Reliance on mass‐specific detection – EI, ESI, and ESI MS/MS  spectra of mitragynine, speciogynine, and speciociliatine are  nearly identical – Interference of speciociliatine – would probably not be detected  by either DAD or MS detectors – Methods should account for interference – derivatization may be necessary to overcome poor chrom. res.