SPADA Draft Documents

https://www.iata.org/publications/dgr/Pages/index.aspx (accessed 15-Apr-2019). These 522 regulations should be consulted before shipping soil by air. 523 Due to the possible presence of invasive species, even if a state does not have 524 regulations or quarantine rules in place, sterilize excess soils and plant material by the 525 autoclave method before disposal in a landfill. 530 method or system will depend on the method or system being tested. General guidance 531 includes testing a minimum of three replicates of each sample and including positive and 532 negative controls. Laboratory experiments also need to include multiple soil types. It is 533 recommended to test at least 5 different soils with the varying characteristics listed in 534 Table 1. Online resources, such as the web soil survey (Table 2), provide tools to search 535 for locations to collect the various soils with the desired approximate pH, clay content 536 and organic content. Academic institutions with soil science departments may also be 537 useful in obtaining specific soils (Table 2). 538 Prior to experimentation, pH, water content, water holding capacity, organic carbon 539 content, texture and CEC of each soil should be measured. Other characteristics that may 540 affect the experiments should also be included. If it is important to start an experiment 541 quickly after sample collection, then soil pH and water content should be measured 542 immediately, and the remaining characteristics measured at a later time. Soil pH and dry 543 weight should be monitored during the course of the experiment as these could affect the 544 526 527 528 529 10.0 Experimental Set-up Recommendations 10.1 Laboratory Test and Evaluation Set-up The laboratory experimental set-up for testing and evaluating a biothreat agent

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