SPADA Draft Documents

using state-of-the-art assay design algorithms to evaluate assay designs and rank assays prior to 174 wet lab testing (detailed below). This approach reduces the number of experimental iterations 175 compared to the traditional approach. The following sections compare and contrast the various 176 steps of the two approaches.

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Figure 4. Traditional vs. modern assay development pipeline.

Assay Design

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Target selection: user defined vs unbiased (in silico)

Traditionally, assay target selection has been an ill-defined process that is strongly influenced 184 by the preferences and experience of individual assay designers. Often, assay targets are selected 185

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