AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC Official Method 2016.14

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Fructans in Infant Formula & Adult/Pediatric Nutritional Formula by Anion-Exchange Chromatography 2 with Pulsed Amperometric Detection after Enzymatic Treatment 3

First Action 2016

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Final Action 2019

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[Applicable to the measurement of fructans in infant formula and adult/pediatric nutritional formula 6

(powders, ready-to-feed liquids and liquid concentrates) from 0.03 g/100 g to 5 g/100 g reconstituted 7

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final product.]

Caution .— The method employs corrosive (sodium hydroxide, acetic acid) and toxic (sodium azide) 9

chemicals. Use appropriate personal protective equipment when performing testing. Refer to the materials 10

safety data sheets and take appropriate additional safety precautions for handling and waste disposal. 11

Table 2016.14A Collaborative study results

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Overall mean, g/100 g b

Horwitz ratio f

RSD r

, %

RSD R

, %

Sample name

n a

SRM 1869 c

11 (1)

0.204

3.78

5.90

1.2

Adult nutritional RTF d (1)

12 (0)

1.28

7.65

12.8

3.3

Adult nutritional RTF d (2)

12 (0)

2.67

3.70

10.5

3.1

Infant formula RTF

12 (0)

0.300

2.27

15.1

3.2

Child formula powder e

12 (0)

0.275

3.80

8.35

1.7

Toddler formula powder milk based e

12 (0)

0.209

4.25

9.94

2.0

1