AOAC OMB Final Action Recommendation (December 2019)-2016.14
2016.14 (October 2019) – FOS-03 METHOD
FOR ERP USE ONLY DO NOT DISTRIBUTE
380
Chromatographic conditions
381
Mobile phase A
: 300 mM NaOH
382
Mobile phase B
: Purified water with resistivity ≥18 MΩ
383
Mobile phase C
: 500 mM NaOAc + 150 mM NaOH
384
Flow rate
: 1.0 mL/min
385
Column
: CarboPac PA1, 250 x 4 mm, 10 µm (ThermoFisher Scientific, or
386
equivalent)
387
Oven temperature
: 30 °C
388
Injection volume
: 25 µL
: Quadruple waveform described in Table 2016.14D , gold working
389
Detector parameters
390
electrode and Ag/AgCl reference electrode (or equivalent).
391
392
393
Elution gradient
:
500 mM NaOAc + 150 mM NaOH, % C
Water,
Flow rate, mL/min
300 mM NaOH, % A
Time, min
% B
0.0
1.0
30.0
60.0
10.0
15.0
1.0
30.0
41.0
29.0
40.0
1.0
16.6
22.7
60.7
21
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