AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

380

Chromatographic conditions

381

Mobile phase A

: 300 mM NaOH

382

Mobile phase B

: Purified water with resistivity ≥18 MΩ

383

Mobile phase C

: 500 mM NaOAc + 150 mM NaOH

384

Flow rate

: 1.0 mL/min

385

Column

: CarboPac PA1, 250 x 4 mm, 10 µm (ThermoFisher Scientific, or

386

equivalent)

387

Oven temperature

: 30 °C

388

Injection volume

: 25 µL

: Quadruple waveform described in Table 2016.14D , gold working

389

Detector parameters

390

electrode and Ag/AgCl reference electrode (or equivalent).

391

392

393

Elution gradient

:

500 mM NaOAc + 150 mM NaOH, % C

Water,

Flow rate, mL/min

300 mM NaOH, % A

Time, min

% B

0.0

1.0

30.0

60.0

10.0

15.0

1.0

30.0

41.0

29.0

40.0

1.0

16.6

22.7

60.7

21