AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

All powdered samples were required to be analysed on a reconstituted basis, using 25 g of material and 200 g of water, with the exception of the Standard Reference Material (SRM 1869) which had to be reconstituted by dissolving 10 g of powder in 90 g of water. The liquid samples were analysed “as is”. For most of the samples, no additional dilution was needed. However, for some samples a dilution factor of 10 had to be applied. A table summarizing the reconstitution rates and dilution factors to be applied was included in the MLT protocol received by the participating laboratories and can be found in Table 1. A practice sample was also included in the kit. The participating laboratories were asked to analyse the practice sample in duplicate and to record and report any deviation from the method described in the protocol. The final result, in g/100 g reconstituted product, as well as sample weights, dilutions, peak areas of standards and sample extracts, glucose and fructose concentrations in the sample extracts and blank measurements, were communicated to the study director by filling the reporting template provided with the protocol. Information on the analytical column and instrument type used, as well as brand of the graphitized carbon column and use or not of Carrez clarification were also required. After review by the study director, the results found to be within a range of expected levels qualified the laboratories for the second part of the study. All qualified participants were asked to analyse the 16 coded MLT samples (one single determination per sample) on 2 days (8 samples per day). Each sample was assigned to either day 1 or day 2, the blind duplicates being assigned to the same day. Results were communicated to the study director by filling a reporting template provided with the protocol. The information to be filled in this template was similar to the one used in part 1 of the MLT.

Table 1. Reconstitution rates and dilution factors to be applied on MLT samples Sample Code Day Reconstitution Rate

Dilution Factor

MLT Sample 1 MLT Sample 2 MLT Sample 3 MLT Sample 4 MLT Sample 5 MLT Sample 6 MLT Sample 7 MLT Sample 8 MLT Sample 9 MLT Sample 10 MLT Sample 11 MLT Sample 12 MLT Sample 13 MLT Sample 14 MLT Sample 15 MLT Sample 16

GBAN911 ACGY733 PTLO721 FJVP596 YEJA706 DPLR873 TSWX969 NSIG474 RQXQ518 EFXN778 URTF231 GVPE615 NSRB999 WKHN288 BFAO941 JSDT587

1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2

10 g + 90 g water

1

As is

10

10 g + 90 g water

1

As is As is As is As is As is

10

1

10 10

1 1 1 1 1 1 1 1 1

25 g + 200 g water 25 g + 200 g water 25 g + 200 g water 25 g + 200 g water 25 g + 200 g water 25 g + 200 g water 25 g + 200 g water 25 g + 200 g water