AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 4. Collaborative study results

Requirements (SMPR 2014.002)

≤ 6 %

≤ 12 %

Overall mean, g/100 g b

RSD r , %

RSD R , %

Horwitz ratio f

Meets SMPR

Sample name

n a

Yes

SRM 1869 c

11 (1) 12 (0) 12 (0) 12 (0) 12 (0) 12 (0)

0.204

3.8 7.7 3.7 2.3 3.8 4.3

5.9

1.2 3.3 3.1 3.2 1.7 2.0

No

Adult nutritional RTF d (1) Adult nutritional RTF d (2) Infant formula RTF Child formula powder e

1.28 2.67

13 11 15

Yes

No

0.300 0.275 0.209

Yes Yes Yes

8.4 9.9

Toddler formula powder milk-based e Infant formula powder FOS/GOS based e Infant formula powder milk based e

10 (2)

0.0308

3.6

12

1.7

12 (0) No a Number of laboratories considered in the evaluation (number of laboratories with data removed as outliers). b Results are reported in g/100 g of product as consumed (reconstituted or RTF) c Reconstitution rate: 10 g product + 90 g water d RTF = Ready-to-feed e Reconstitution rate: 25 g product + 200 g water f HorRat is not necessary applicable to methods with indefinite analytes like polymers [12] such as fructans 0.264 5.3 15 3.0

Table 5. Results per sample and laboratory (samples 1 to 8) a

Adult nutritional RTF (1)

Adult nutritional RTF (2)

SRM 1869

Infant formula RTF

Lab code

Sample 1 0.217 0.278 b 0.194 0.213

Sample 3 0.214 0.251 b 0.188 0.215

Sample 2

Sample 7

Sample 4

Sample 6

Sample 5

Sample 8

1 2 3 4 5 6 7 8 9

1.32 1.66 1.19 1.36 1.11 1.31 1.25 1.25 1.32 1.27 1.15 1.13

1.33 1.59 1.23 1.20 0.92 1.27 1.39 1.61 1.28 1.26 1.20 1.21

2.75 2.98 2.52 2.92 2.15 2.87 2.90 2.65 2.61 2.80 2.38 2.59

2.82 2.99 2.46 3.18 1.98 2.57 2.94 2.61 2.72 2.72 2.45 2.48

0.337 0.310 0.221 0.367 0.226 0.322 0.341 0.303 0.324 0.302 0.268 0.289

0.338 0.302 0.220 0.355 0.209 0.326 0.338 0.307 0.331 0.307 0.281 0.271

Unable to report results

0.195 0.195 0.207 0.209 0.204 0.211 0.191 0.195

0.178 0.215 0.222 0.208 0.212 0.216 0.205 0.187

10 11 12 13 14

Unable to report results

a Total Fructans results in g/100 g product as consumed (reconstituted or RTF) b Outlier according to Single Grubb’s Test