AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 9. Elution program used by lab 6

Flow rate, mL/min

400 mM NaOH, % A

Water, % B

Time, min

0.0

1.0 1.0 1.0 1.0 1.0 1.0 1.0

2 2

98 98 75 25 25 98 98

12.5 30.3 30.4 42.9 43.0 50.0

25 75 75

2 2

a) Standard solution Level 4

b) Child formula powder

Figure 3. Example of chromatograms obtained by lab 6, using PA10 column without post-column addition of NaOH and elution program described in Table 9