AOAC OMB Final Action Recommendation (December 2019)-2016.14
2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE
Table 12. Elution program used by lab 8
Flow rate, mL/min
Time, min
Mobile phase
0
1.3 1.3 1.3 1.3 1.3 1.3
10.5 mM NaOH 10.5 mM NaOH
20 21 24 25 35
150 mM NaOH + 250 mM NaOAc (washing) 150 mM NaOH + 250 mM NaOAc (washing)
10.5 mM NaOH 10.5 mM NaOH
a) Standard solution Level 4
b) Child formula powder
Figure 5. Example of chromatograms obtained by lab 8, using PA1 column (4 x 250 mm) without post- column addition and elution program described in Table 12
Child formula powder Figure 6. Example of chromatogram obtained by lab 11, using PA1 column without post-column addition
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