AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 12. Elution program used by lab 8

Flow rate, mL/min

Time, min

Mobile phase

0

1.3 1.3 1.3 1.3 1.3 1.3

10.5 mM NaOH 10.5 mM NaOH

20 21 24 25 35

150 mM NaOH + 250 mM NaOAc (washing) 150 mM NaOH + 250 mM NaOAc (washing)

10.5 mM NaOH 10.5 mM NaOH

a) Standard solution Level 4

b) Child formula powder

Figure 5. Example of chromatograms obtained by lab 8, using PA1 column (4 x 250 mm) without post- column addition and elution program described in Table 12

Child formula powder Figure 6. Example of chromatogram obtained by lab 11, using PA1 column without post-column addition