AOAC OMB Final Action Recommendation (December 2019)-2016.14
FOS-03 FOR WORKING GROUP/ERP USE ONLY DO NOT DISTRIBUTE
Therefore,
CVA
s
1
1
C
p k
5. 412
1 mA
s
Where:
C = Concentration of fructan in the sample in mg/100g
A 1 = Area of sample fructose peak
A s = Area of standard fructose peak
V 1 = Volume of sample solution (in mL), normally 100 mL
m 1 = weight of sample used to prepare the sample solution (in mg), normally 1000 mg
C s = Concentration of fructose standard (in μg/mL), normally around 7 μg/mL
k = correction factor for water uptake
p = correction factor for loss of terminal monosaccharide residue.
0.2
=
volume of “sample filtrate” taken for hydrolysis of sucrose and starch (in mL)
1.1
=
total volume of “solution A” (in mL)
0.4
=
aliquot of “solution A” taken for SPE cleanup (in mL)
0.3
=
volume in which dried fructans are resuspended for digestion (in mL)
100
=
conversion factor to express final result in mg/100 g
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