AOAC OMB Final Action Recommendation (December 2019)-2016.14

FOS-03 FOR WORKING GROUP/ERP USE ONLY DO NOT DISTRIBUTE

Therefore,

CVA

  s

1

1

C

p k



5. 412 

1 mA



s

Where:

C = Concentration of fructan in the sample in mg/100g

A 1 = Area of sample fructose peak

A s = Area of standard fructose peak

V 1 = Volume of sample solution (in mL), normally 100 mL

m 1 = weight of sample used to prepare the sample solution (in mg), normally 1000 mg

C s = Concentration of fructose standard (in μg/mL), normally around 7 μg/mL

k = correction factor for water uptake

p = correction factor for loss of terminal monosaccharide residue.

0.2

=

volume of “sample filtrate” taken for hydrolysis of sucrose and starch (in mL)

1.1

=

total volume of “solution A” (in mL)

0.4

=

aliquot of “solution A” taken for SPE cleanup (in mL)

0.3

=

volume in which dried fructans are resuspended for digestion (in mL)

100

=

conversion factor to express final result in mg/100 g