AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

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AOAC Official Method 2016.14 1 Fructans in Infant Formula & Adult/Pediatric Nutritional Formula by Anion-Exchange 2 Chromatography with Pulsed Amperometric Detection after Enzymatic Treatment 3 First Action 2016 4 Final Action 2019 5 [Applicable to the measurement of fructans in infant formula and adult/pediatric nutritional formula 6 (powders, ready-to-feed liquids and liquid concentrates) from 0.03 g/100 g to 5 g/100 g 7 reconstituted final product.] 8 Caution .— The method employs corrosive (sodium hydroxide, acetic acid) and toxic (sodium azide) 9 chemicals. Use appropriate personal protective equipment when performing testing. Refer to the 10 materials safety data sheets and take appropriate additional safety precautions for handling and 11 waste disposal. 12 Table 2016.14A Collaborative study results 13

Overall mean, g/100 g b

RSD r

,

RSD R

,

Horwitz ratio f

n a

Sample name

%

%

SRM 1869 c

11 (1) 12 (0) 12 (0) 12 (0) 12 (0)

0.204

3.78 7.65 3.70 2.27 3.80 4.25

5.90 12.8 10.5 15.1 8.35 9.94

1.2 3.3 3.1 3.2 1.7 2.0

Adult nutritional RTF d (1) Adult nutritional RTF d (2) Infant formula RTF Child formula powder e

1.28 2.67

0.300 0.275 0.209

Toddler formula powder milk based e 12 (0)

Infant formula powder FOS/GOS based e Infant formula powder milk based e

10 (2)

0.0308

3.60

11.7

1.7

12 (0) 3.0 a Number of laboratories considered in the evaluation (number of laboratories with data removed as outliers). 14 b Results are reported in g/100 g of product as consumed (reconstituted or RTF). 15 c Reconstitution rate: 10 g product + 90 g water. 16 d RTF = Ready-to-feed. 17 e Reconstitution rate: 25 g product + 200 g water. 18 f HorRat is not necessary applicable to methods with indefinite analytes like polymers [12] such as fructans. 19 0.264 5.26 14.6

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