AOAC OMB Final Action Recommendation (December 2019)-2016.14
2016.14 (October 2019) – FOS-03 MANUSCRIPT
FOR ERP USE ONLY DO NOT DISTRIBUTE
1
(m) Membrane filter nylon. —0.2 µm, Ø 4.7 cm (only when using PA1 column for HPAEC-
2
PAD).
3
(n) Analytical column. —CarboPac PA20 (150 x 3 mm, 6.5 µm) with no guard, or CarboPac
4
PA1 (250 x 2 mm, 10 µm), with PA1 guard (50 x 2 mm, 10 µm) (Thermo Fisher Scientific,
5
MA USA), or equivalent.
6
(o) Metal free liquid chromatograph consisting of :
7
- Quaternary gradient pump able to deliver a flow of 0.5 mL/min (PA20) or 0.25 mL/min
8
(PA1).
9
- Helium degassing system.
10
- Autosampler (cooling system recommended) able to inject 25 µL (PA20) or 20 µL (PA1).
11
- Column compartment able to maintain a temperature of 30 °C (PA20) or 20 °C (PA1).
12
- Electrochemical detector working in pulsed amperometric detection mode, equipped with
13
a gold working electrode and an Ag/AgCl reference electrode (or equivalent).
14
- Isocratic pump for post-column delivery, with pulse dampener, 125-µL reaction coil (or
15
equivalent) and T-piece.
16
- Data acquisition system.
17
C.
Chemicals and Standards
18
(a) Deionised Water. —Purified with resistivity ≥18 MΩ .
19
(b) Maleic acid. —Puriss., ≥ 99.0 %.
20
(c) Acetonitrile .—Gradient grade for liquid chromatography.
21
(d) Acetic acid .—Glacial 100 % anhydrous, GR for analysis.
22
(e) Potassium cyanohexaferrate(II)trihydrate .—[Optional].
23
(f) Zinc acetate .—[Optional].
24
(g) Trifluoroacetic acid (TFA) .—99 %.
25
(h) Hydrochloric acid .—1 M .
26
(i) Sodium acetate .—Anhydrous, ≥99.0 % (only when using PA1 column for HPAEC-PAD).
9
Made with FlippingBook HTML5