AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

FOR ERP USE ONLY DO NOT DISTRIBUTE

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L. 2 Use bracketed calibration by injecting 3 standards followed by 10 samples, and repeating this 3 process (e.g., inject standards at levels 1, 3 and 5 then 10 samples; inject standards at levels 2, 4 4 and 6 then 10 samples; inject standards 1, 3, 5, etc.). For each analyte (glucose and fructose) use 5 the instrument software to plot a six-point standard curve of [Instrument response (area) for 6 analyte] / [Instrument response (area) for internal standard] against the concentration of the analyte 7 in the standard. Fit a quadratic curve to the data without forcing through zero. Use the calibration 8 curve to calculate the glucose and fructose concentrations in Solution B. For the blank 9 measurements, ignore any signals if they are not greater than 10x the signal-to-noise ratio. 10 (a) Calculate the glucose released from fructans, C G , in g/100 g RTF or reconstituted product 11 (sample preparation according to H(b) ) or powder product (sample preparation according 12 to H(c)), as follows: 13 = × × � � × 0.0001 14 (b) Calculate the fructose released from fructans, C F , in g/100 g RTF or reconstituted product 15 (sample preparation according to H(b) ) or powder product (sample preparation according 16 to H(c)), as follows: 17 = × × � � × 0.0001 18 (c) Calculate the total fructan content, TF , in g/100 g RTF or reconstituted product (sample 19 preparation according to H(b) ) or powder product (sample preparation according to H(c)), 20 as follows: 21 = ( × 0.9) + 22 where C GB = glucose concentration in Solution B (in µg/mL), C FB = Fructose 23 concentration in Solution B (in µg/mL), D = dilution factor between Solution A and 24 Calibration & Calculations

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