AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

FOR ERP USE ONLY DO NOT DISTRIBUTE

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Elution gradient:

500 mM NaOAc + 150 mM NaOH, % C

Flow rate, mL/min

300 mM NaOH, % A

Water, % B

Time, min

0.0

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0

30.0 30.0 16.6

60.0 41.0 22.7

10.0 29.0 60.7

15.0 40.0 40.1 45.0 45.1 50.0 50.1 60.0

0.0 0.0

0.0 0.0 0.0 0.0 0.0

100.0 100.0

100.0 100.0

0.0 0.0

30.0 30.0

10.0 10.0

60.0

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Example of chromatograms:

4 In black, chromatogram of untreated sucrose/FOS solution. In blue, sucrose/FOS solution treated 5 with a sucrase enzyme mixture performing as expected in the studied conditions. The sucrose has 6 been completely hydrolysed whereas the alteration of the FOS ingredient profile is minimal. 7

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