AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

FOR ERP USE ONLY DO NOT DISTRIBUTE

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Table 2. Results of practice sample a

Repetition with sucrase mixture (batch C)

Result of 1 st analysis

Batch of sucrase mixture

Lab code

Mean, g/100 g

Mean, g/100 g

1 2 3 4 5 6 7 8 9

C A

0.265 0.292 0.219 0.237 0.259 0.259 0.210 0.270 0.232 0.273 0.267 0.249 f

B b

0.255

C

Unable to report results

B b B b B b

0.277 c 0.278

Not repeated

A

10 11 12 13 14

Unable to report results

B b

0.284 d

? e

B b B b

Not repeated 0.246 – 0.252 g

a Results are reported in g/100 g of product as reconstituted (25 g product + 200 g water). 2 b Batch B is the unsatisfactory batch of sucrase enzyme mixture. 3 c One replicate analysed with MLT samples Day 1 and a second replicate with Day 2. 4 d Single analysis. 5 e Batch unknown but according to the purchasing date, expected to be a satisfactory batch. 6 f Blank measurements not performed. 7 g Analysis repeated twice, once with MLT samples Day 1 and once with MLT samples Day 2. 8 9 Table 3. Statistical evaluation of results from determination of fructans in the practice 10 sample a 11

Requirements (SMPR 2014.002)

≤ 6 %

≤ 12 %

Sample name

n b

Mean, g/100 g

RSD r

, % RSD R

, %

Child formula powder d Child formula powder e

9 c

0.266 d 0.242 e

2.53 d 1.63 e

6.70 d 9.16 e

7

12

a Results are reported in g/100 g of product as reconstituted (25 g product + 200 g water). 13 c Lab 11 eliminated because of single analysis. Lab 14 data obtained on Day 1 taken into account. 14 d Results obtained with satisfactory batches of sucrase enzyme mixture. 15 e Results obtained with unsatisfactory batch (B) of sucrase enzyme mixture. 16 b Number of laboratories considered in the evaluation.

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