AOAC OMB Final Action Recommendation (December 2019)-2016.14
2016.14 (October 2019) – FOS-03 MANUSCRIPT
FOR ERP USE ONLY DO NOT DISTRIBUTE
Blank measurements 1 According to the experience gained through the Single Lab Validation (SLV) [5] and as 2 described in the method, “a blank measurement is only necessary for some matrices and may be 3 skipped if it has already been established that the blank measurement has a negligible impact on 4 the result of a given matrix”. As all samples in this study were unknown, participating laboratories 5 made blank measurements for every sample. The variations of the blank measurements between 6 the participants were high and it was questionable if the signals in the blanks were of sufficient size 7 to be above the limit of quantitation (LoQ). The mean results with blank measurements subtracted 8 were compared with the mean results obtained without subtracting blank measurements using an 9 unpaired t-test. As can be seen from the p-values depicted in Table 7 , the mean results of the 10 samples are not statistically different, whether the blank measurement is subtracted or not. This 11 demonstrates that for these matrices a blank subtraction was probably not necessary. 12 13 Table 7. Comparison of results with and without blank subtraction 14
Mean with blank subtraction, g/100 g b
Mean without blank subtraction, g/100 g b
P- value c
Significantly different ?
Sample name
n a
SRM 1869 d
12 12 12 12 12 12 12 12
0.209
0.217
0.27
No No No No No No No
Adult nutritional RTF e (1) Adult nutritional RTF e (2) Infant formula RTF Child formula powder f
1.28 2.67
1.33 2.78
1.3 2.7
0.300 0.275 0.209 0.032 0.264
0.315 0.286 0.219 0.032 0.278
0.41 0.20 0.18 0.78 0.30
Toddler formula powder milk-based f Infant formula powder FOS/GOS based f Infant formula powder milk based f
No a Number of laboratories considered in the evaluation. All data have been used for the calculation, no outliers removed. 15 b Results are reported in g/100 g of product as consumed (reconstituted or RTF). 16 c T-test 2 sided (hyp=unpaired, equal variance) 17 d Reconstitution rate: 10 g product + 90 g water. 18 e RTF = Ready-to-feed 19 f Reconstitution rate: 25 g product + 200 g water.
20 21 22
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