AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

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Table 9. Elution program used by lab 6

Flow rate, mL/min

400 mM NaOH, % A

Water, % B

Time, min

0.0

1.0 1.0 1.0 1.0 1.0 1.0 1.0

2 2

98 98 75 25 25 98 98

12.5 30.3 30.4 42.9 43.0 50.0

25 75 75

2 2

2 3 4

B

A

5 Figure 3. Example of chromatograms obtained by lab 6, using CarboPac PA10 column 6 without post-column addition of NaOH and elution program described in Table 9. A: 7 Calibration standard solution. B: Child formula powder containing fructans.

8 9

10 11 12 13

40