AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

FOR ERP USE ONLY DO NOT DISTRIBUTE

B

A

1 Figure 4. Example of chromatograms obtained by lab 7, using Metrosep Carb 2 column 2 without post-column addition of NaOH and flow gradient described in Table 10. A: 3 Calibration standard solution. B: Child formula powder containing fructans.

4 5 6 7 8 9

Table 12. Elution program used by lab 8

Flow rate, mL/min

Time, min

Mobile phase

0

1.3

10.5 mM NaOH

20

1.3

10.5 mM NaOH

21

1.3

150 mM NaOH + 250 mM NaOAc (washing)

24

1.3

150 mM NaOH + 250 mM NaOAc (washing)

25

1.3

10.5 mM NaOH

35

1.3

10.5 mM NaOH

10 11

42